بررسی بیان ژن های GPX3و NRF2 در اووسیتهای موش مدل تخمدان پلی کیستیک تحریک تخمک گذاری شده

Background: One of the common ovary malfunction is polycystic ovary syndrome (PCO). In this study, GPX3 and NRF2 gene experssion which are responsible for oocyte maturation, were studied. Methods: Adult female mice were divided into groups of control, sham, PCO, hyperstimulated control, hyperstimulated sham and hyperstimulated PCO. During two weeks, the weight of all mice and the level of LH and FSH hormones were evaluated. In hyperstimulated groups, after 14 hours of hyperstimulation, the oocytes were collected from fallopian tube and the expression of GPX3 and NRF2 genes were evaluated by real time PCR. Ovary tissues from all groups were H&E stained and evaluated by morphometric methods. The results were analyzed by SPSS software, ANOVA and tukey tests. Results: Morphometric studies showed many cystes in cortex and medulla of the ovary in PCO group. In PCO group the level of LH and FSH hormones were higher than control and sham groups significantly (p<0.05), but in hyperstimulated groups, they were not significant. The GPX3 and NRF2 gene experssion in hyperstimulated PCO group were significantly lower than hyperstimulated control and sham groups (p<0.05). Conclusion: The results of this study shows that the time of oocyte maturation in hyperstimulated PCO group is different when compared to the other hyperstimulated groups. &nbsp;